The 300SB-C8 is a high-performance microparticulate 300Å C8 packing reversed phase column from Agilent that can be used at low pH for intact protein analysis and peptide mapping.
The Agilent ZORBAX Rapid Resolution High Definition (RRHD) 300SB-C8 threaded columns are specifically designed for use with ultra-high performance liquid chromatographs (UHPLCs). They feature a high-performance microparticulate 300Å C8 packing with a sterically protected stationary phase to ensure chemical stability and long column life, particularly in aggressive mobile phases.
| Maximum Operating Pressure | 1200 bar |
|---|---|
| Particle Pore Size | 300Å |
| Maximum Recommended Temperature | 80 °C |
| Compatible Mobile Phase pH Range | pH 0.8 to 8.0 |
Long-term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.
Yes, they are especially suited for applications using high-sensitivity detectors that require low backgrounds, such as mass spectrometers.