This application demonstrates a reversed-phase separation using high-organic concentration (>90% methanol) on a ZORBAX XDB-C8 column. By using reversed-phase chromatography for water-soluble and fat-soluble vitamins, labs requiring both assays can now minimize changeover time.
This document describes an application for analyzing fat-soluble vitamins (A, D, E, and K) found in fortified foods like margarine, milk products, and infant formulas. The method utilizes reversed-phase chromatography on a ZORBAX XDB-C8 column with high organic concentration (>90% methanol), replacing traditional normal-phase chromatography to minimize changeover time for labs performing both water-soluble and fat-soluble vitamin assays.
| Column Dimensions | 4.6 x 150mm |
|---|---|
| Column Part Number | 993967-906 |
| Mobile Phase Ratio | 5:95 water : MeOH |
| Injection Volume | 5 SpL |
| Flow Rate | 1 mL/min |
| Detection Wavelength | 280 nm |
| Operating Temperature | Ambient to 50°C |
The application demonstrates a reversed-phase separation using high-organic concentration on a ZORBAX XDB-C8 column.
The column used in this method has dimensions of 4.6 x 150mm.
The mobile phase consists of a 5:95 ratio of water to methanol.