This application note details the relative merits of two techniques for the analysis of organotin compounds: gas chromatography-inductively coupled plasma mass spectrometry (GC-ICP-MS) and high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS).
The document details an analytical study comparing GC-ICP-MS and HPLC-ICP-MS techniques for the determination of organotin compounds in various matrices, including sediments, seafood, and manufactured products. It highlights that while GC allows simultaneous multi-elemental speciation analysis after derivatization, HPLC offers advantages by eliminating the need for time-consuming derivatization steps.
The study utilizes an Agilent 7500i ICP-MS detector coupled with both an Agilent 1100 HPLC system and an Agilent 6890 GC. The text describes specific experimental procedures, including accelerated solvent extraction using a Dionex ASE 200 system, sample derivatization for GC analysis, and the implementation of isotope dilution mass spectrometry (IDMS) to reduce measurement uncertainties.
| Plasma gas flow (HPLC-ICP-MS) | 14.5-14.9 L min' |
|---|---|
| Carrier gas flow (HPLC-ICP-MS) | 0.65-0.75 L min' |
| RF power (HPLC-ICP-MS) | 1350-1550 W |
| Plasma gas flow (GC-ICP-MS) | 14.5-14.9 L min' |
| Carrier gas flow (GC-ICP-MS) | 0.80-0.85 L min' |
| RF power (GC-ICP-MS) | 1100-1200 W |
Each cell was extracted using five 5-minute cycles at 100 °C and 1500 psi after a 2-minute preheat and 5-minute heat cycle.
The sample solution is shaken for 10 minutes with hexane after being derivatized with sodium tetra-ethylborate.