High-Speed Separation of Water-Soluble Vitamins with Ion-Pairing Chromatography
This document details a method for the rapid separation and detection of water-soluble vitamins, including four B vitamins, folic acid, and vitamin C. The analysis is performed using reverse-phase ion-pairing chromatography on a 75 mm ZORBAX SB-C8 column, achieving results in less than 5 minutes.
| Column | ZORBAX SB-C8 (4.6 x 75 mm, 3.5 um) |
|---|---|
| Mobile Phase | 10mM Hexane Sulfonate with 0.1% Phosphoric Acid:MeOH (74:26) |
| Injection Volume | 10 pL |
| Flow Rate | 1 mL/min |
| Detection Wavelength | UV(245 nm) |
| Analysis Time | Less than 5 minutes |
The method analyzes four B vitamins, folic acid, and vitamin C.
The analysis of these vitamins is completed in less than 5 minutes.
The specified column has dimensions of 4.6 x 75 mm.