Agilent Application Note: Separation of Retinal Isomers via HPLC

About this manual

This document describes an application for High-Performance Liquid Chromatography (HPLC) used to separate various Vitamin A isomers, including retinal and retinol. It highlights the use of ZORBAX Sil silica columns as a more friendly solvent alternative to traditional 1,4-dioxane methods.

What's inside

• Application
• HPLC separation
• Retinal Isomers
• Mobile-Phase Composition
• ZORBAX Sil
• Chromatogram Data

Frequently asked questions

What is the recommended column size for separating retinal isomers?

The document specifies a ZORBAX Sil column with dimensions of 4.6 x 250 mm.

What injection volume and temperature are used in the described procedure?

The method requires an injection volume of 20pL at a temperature of 25°C.

Which mobile phase combination is listed for separating all-trans retinal?

A mixture of n-hexane and BME in a ratio of 7:3 is used to separate all-trans retinal.