Agilent Analysis of Essential Oil Compounds Using Retention Time Locked Methods Application Note

About this manual

This document describes two retention time locked methods for the analysis of essential oil compounds. The first method utilizes a gas chromatography/flame ionization detector (GC/FID) with a 50 m x 320 µm id x 1.05 µm HP-5 column, while the second employs a GC/mass spectrometry (GC/MS) approach using a 30 m x 250 µm id x 0.25 µm HP-5MS column.

The text details the experimental conditions for both methods, including specific oven programs and carrier gas settings adjusted via retention time locking to ensure reproducible absolute retention times. It explains how these databases can be used to automatically search for flavor compounds in food extracts or essential oil constituents based on retention times and mass spectra.

What's inside

• Abstract
• Introduction
• Experimental GC/FID Conditions
• GC/MS Conditions
• Results and Discussion GC/FID Method
• Identification of Main Compounds

Frequently asked questions

What is the retention time for n-pentadecane in the GC/FID method?

The inlet pressure is adjusted to give a retention time of exactly 70.000 min for n-pentadecane.

What is the retention time for n-pentadecane in the GC/MS method?

The inlet pressure is adjusted to give a retention time of exactly 27.500 min for n-pentadecane.

What solvent is used to prepare the test mixtures?

Test mixtures are prepared from pure chemicals at 0.1% concentration in carbon tetrachloride or chloroform.