Agilent Separation of Water-Soluble Vitamins Using Reversed-Phase Chromatography Application Note

About this manual

The document details an alternative to routine isocratic separation for analyzing water-soluble vitamins in the pharmaceutical industry. It presents a method using reversed-phase gradient chromatography without ion pairing to separate an eight-component sample containing B vitamins, pantothenic acid, folic acid, and vitamin C.

What's inside

• Food Analysis
• Reversed-Phase Gradient
• ZORBAX SB-C8 Column
• Mobile Phase Conditions
• Injection Parameters
• Detection Method

Frequently asked questions

Which column is used for the analysis of water-soluble vitamins?

A ZORBAX SB-C8 column with dimensions of 4.6x 150 mm is used for the analysis.

What is the injection volume and flow rate for the procedure?

The procedure requires an injection of 10 pL and a flow rate of 1 mL/min.

At what wavelength is the detection performed?

Detection is performed using UV at a wavelength of 245 nm.