Agilent Manuals - Page 141

Agilent manuals, page 141

• Agilent Ulcer Medications

  This Application Note describes the basic principles and conditions of using Agilent ZORBAX StableBond columns to separate ulcer drugs.

• Agilent Separation of Hypericin in St. John\

  This document describes a method for detecting Hypericin and provides an application example of the method.

• Agilent Customer States That Performance of ZORBAX Columns is \"Superior\"

  This document introduces ZORBAX SB-CN column, including that it is the only acid-stable CN column and has unique selectivity.

• Agilent Stability of Short-Chain Bonded Phases

  This file introduces StableBond short-chain bonded-phase columns from Agilent Technologies, which are much more stable than standard packings and ideal alternatives to C- 18 columns for stable reversed-phase method development.

• Agilent ZORBAX SB-C18 Stability

  ZORBAX SB-C18 is the most rugged, stable commercial C18 column produced by Agilent Technologies.

• Agilent Calibration of the ZORBAX GF-250 Column with Globular Proteins

  This document describes the characteristics and usage of the ZORBAX GF-250 column. The column has a wide separation range and is suitable for separating proteins with different PI and hydrophobicity.

• Agilent Effect of Organic Modifier pH on Selectivity

  pH control is very important in providing separation stability for polar organic compounds that are basic or acidic. The short-chain SB-CN column is very stable under low pH conditions (e.g. pH 2.5).

• Agilent Effect of Silica-Support Type - Reversed-Phase Separation of Tricyclic Antidepressants

  This document describes the use of highly purified, less-acidic Type-B silica support (ZORBAX Rx-SIL) in reversed-phase separation of tricyclic antidepressants, which resulted in improved band shape and column efficiency for these basic drugs. Reduced retention for column with Type-B silica is due to lower surface area of its support.

• Agilent Lot-to-Lot Reproducibility - Amphetamines

  This document describes the high reproducibility of the Agilent ZORBAX Rx-C8 column in the detection of basic drugs and introduces the strict quality control in the production process of the column.

• Agilent Lot-to-Lot Reproducibility - ZORBAX SB-C8

  This document introduces the lot-to-lot reproducibility test of Agilent ZORBAX SB-C8 chromatography column products

• Agilent Effect of Particle Size on Sample Load - Theoretical Plates for 5 m 3.5 m Particles

  This document introduces the characteristics of ZORBAX 3.5-µm silica, which offers higher sample load (over 5-µm particles) with comparable column efficiency.

• Agilent Comparison of Column Retentivity vs. Bonded-Phase Chain-Length - Triton X-114

  SB-C3 is a shorter-chain bonded phase that is less retentive than SB-C8 but more retentive than a non-sterically protected TMS phase. SB-C3 can be used to decrease solute retention and is more stable than the non-sterically protected TMS phase.

• Agilent Narrow Bore vs. Standard Bore - Separation of Polypeptides

  This document highlights the advantages and characteristics of narrow bore and standard bore separation of polypeptides. It mentions that narrow bore columns require only 20% of the mobile phase and 1/3 of the sample volume compared to standard bore columns under sample-limited conditions. It also emphasizes the need to minimize extra-column volume for optimal efficiency when using narrow bore columns.

• Agilent Stability-Test Comparison - ZORBAX Eclipse XDB-C8 vs. Symmetry-C8

  This article compares the stability test results of ZORBAX Eclipse XDB-C8 and Symmetry-C8. The ZORBAX particle technology results in an extremely strong and dense silica support that dissolves more slowly at higher pH than xerogel particles used in Symmetry column packings. The extra dense bonding of ZORBAX XDB-C8 helps to shield the silica support at intermediate pH and decreases loss of chromatographic performance compared to Symmetry C8. By combining dense, durable particles, extra-dense bonding, and double endcapping, ZORBAX Eclipse packings (e.g., XDB-C8) have increased lifetime compared to Symmetry columns.

• Agilent Technologies Effect of Particle Size on Column Efficiency - ZORBAX 300SB-C8

  The use of a column packing having reduced particle size can improve efficiency of a separation or, alternatively, increase loading capacity.

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