Agilent Manuals

The use of a column packing having reduced particle size can improve efficiency of a separation or, alternatively, increase loading capacity.

This document compares the use of micro and regular flow cells with ZORBAX 300SB-C8 (3.5 µm) column for peptide and protein analysis. It highlights the reduction of analysis time without compromising efficiency. By reducing the flow cell size, the effects of extra-column volume are minimized with small particle-size packing.

This article discusses the effect of Rx silica, eXtra-Dense Bonding, and double end capping on peak shape, as well as the improved chromatographic performance of Eclipse XDB column packings for basic molecules (P and A) at neutral pH. By reducing the interaction of basic molecules with the packing surface, good peak shape is achieved. ZORBAX XDB-C8 exhibits better peak shape than Symmetry C8, even for the strongly basic molecule amitriptyline.

The use of smaller particle-size columns can increase resolution and peak height, while decreasing run time.

This is a list of OSHA methods for analysis using Zorbax HPLC columns. These lists come from the OSHA web site www.osha-slc.gov/, under 'Chemical Sampling Information'. The document describes standard methods for analysis of many compounds using Zorbax HPLC columns.

Using smaller-diameter columns can improve instrument sensitivity (peak response), save time and reduce solvent consumption

This document introduces the stability of ZORBAX Eclipse XDB-C8 column to neutral pH conditions, and verifies in experiments that ZORBAX Eclipse XDB-C8 column can be used for a longer time in neutral pH conditions.

This document introduces the features of Agilent's StableBond bonded phases, including low pH, high temperature compatibility, and a wide range.

This application note demonstrates the advantages of using high temperature and low pH conditions with ZORBAX StableBond C8 packing materials for high-efficiency preparative separations.

LC/MS columns have outstanding separation and fast equilibration time in short columns, small particles (3.5 vs. 5 µm), gradient mode.

This article introduces the features and characteristics of Agilent ZORBAX Eclipse XDB series products. The series is designed for optimal lifetime at intermediate pH range.

This article describes the use of short column lengths for high performance liquid chromatography (HPLC) and discusses some of the advantages of this method. Short column lengths can reduce analysis times, increase peak heights, and allow for some tolerable loss in resolution. This method is particularly well suited for high-throughput analysis and can reduce solvent consumption and enhance sensitivity for mass-limited samples.