Agilent manuals, page 136
Agilent Purity check of a synthetic alpha-homo polylysine preparation
This document introduces a method for detecting the purity of synthetic �-homo polylysine (250) using capillary electrophoresis. Experimental results show that the purity of synthetic �-homo polylysine (250) in 50 mM phosphate buffer (pH 2.0) solution is greater than 99.7%.
Agilent Isolation purification of reaction byproducts using the Agilent 1100 Series purification system
This Application Note illustrates how to isolate and purify reaction byproducts using the Agilent 1100 Series purification system (AS) and demonstrates that large-scale pooling can be elegantly facilitated by incorporating the funnel tray into the Agilent 1100 Series fraction collector AS.
Agilent Isolation of formononetin other phytoestrogens from red clover with the Agilent 1100 Series purification system
This application note describes how to isolate active natural products from red clover using the Agilent 1100 Series purification systems.
Agilent 1100 Series purification system Manual
This Application Note describes how an evaporative light scattering detector (ELSD) can be used with the Agilent 1100 Series purification system for peak-based fraction collection. The system setup is described, and an example is shown of how an ELSD can be used with the Agilent 1100 Series purification system for peak-based fraction collection.
Agilent Technologies Ergosterol Application Note
This document describes the analysis method for ergosterol, a pro-vitamin of Vitamin D2, in soil extracts. Ergocalciferol (Vitamin D2) is produced by the ultraviolet irradiation of ergosterol in plants, fungi, and yeasts. The use of Agilent ZORBAX Rapid-Resolution Rx-C18 columns enables fast analysis of soil extracts with good peak shape and retention.
Agilent Technologies Catecholamines Application Note
This document describes the method for catecholamine analysis using Agilent ZORBAX SB-C18 column
Agilent Separation of Deoxy Ribonucleosides: ZORBAX SB-C8
This document introduces a high-efficiency method for separating nucleosides using reversed-phase separation with protected-bonded-phase columns.
Agilent Effect of TFA Concentration on the Reversed-Phase Separation of Peptides
Agilent Effect of Bonded Phase Type on a Peptide Separation
Significant differences in selectivity can be achieved by changing the ZORBAX StableBond phase. Note selectivity and resolution differences for peptides S1 and S2. Peptides S1-S5 are decapeptides that differ in amino acids at two locations, causing only slight differences in hydrophobicity.
Agilent Effect of Bonded Phase on Selectivity 10 Polypeptides Separated By Wide-Pore Reversed Phase
This document describes how to improve separation by taking advantage of different bonded-phase chemistries. When using StableBond sterically protected phases, both long-chain and short-chain bonded phases are stable.
Agilent Effect of Column Aging with Acidic Mobile Phase: Silanol-Sensitive Peptide Separation pH 7
This document introduces the effect of column aging with acidic mobile phase on ZORBAX 300SB-C8 (4.6 x 150 mm) (Agilent P/N: 883995-906) column.
Agilent Separation of Peptide Mixture at pH7: Comparison with Commercial Column
Agilent Small Peptides Column Selectivity Comparison
Agilent Bonded-Phase Selectivity - Separation of Polypeptides on 80Å StableBond
This document introduces the separation results of Agilent Technologies' SB-C18, SB-C8, SB-C3, and SB-CN columns at 0-70% B in 36 minutes, and compares them with the separation results on 300Å StableBond columns.
Agilent Effect of Elevated Temperature on Resolution Retention of Polypeptides
Using elevated temperatures for the reverse-phase separation of peptides and proteins can increase column efficiency, change selectivity, and improve peak shape.