This document introduces the characteristics of ZORBAX 3.5-µm silica, which offers higher sample load (over 5-µm particles) with comparable column efficiency.
This document is an application note titled "Effect of Particle Size on Sample Load: Theoretical Plates for 5-µm and 3.5-µm Particles" by Robert Ricker. It compares the performance of Agilent ZORBAX SB-C8 columns with 3.5 µm and 5 µm particle sizes.
ZORBAX 3.5-um silica offers higher sample load while maintaining comparable column efficiency.
Sample viscosity narrows the difference in efficiency observed for the two particle sizes.