This file introduces StableBond short-chain bonded-phase columns from Agilent Technologies, which are much more stable than standard packings and ideal alternatives to C- 18 columns for stable reversed-phase method development.
This document highlights the superior stability of sterically protected short-chain bonded phases, such as CN and C8, compared to standard packings. It specifically features the StableBond column technology as an ideal alternative for stable reversed-phase method development.
The text includes a chart demonstrating the stability of these phases under rigorous testing conditions. The stability testing involved 96 hours of multiple gradients alternating with isocratic runs using 0.1% TFA in water and acetonitrile.
| Column Dimensions | 4.6 x 150 mm |
|---|---|
| Flow Rate | 1 mL/min |
| Stability Testing Duration | 96 hrs |
| Mobile Phase A | 0.1% TFA in water |
| Mobile Phase B | 0.1% TFA in ACN |
The testing involved multiple gradients from 0% B to 100% B in 80 minutes alternated with isocratic runs of 50%A/50%B for 40 minutes.
The test used columns measuring 4.6 x 150 mm with a flow rate of 1 mL/min.