Agilent Manuals - Page 137

ManualLib has 5,354 Agilent manuals, in categories such as Other software, medical technology, Other products, PRBS Generator. Every file can be read online or downloaded as a PDF for free.

Agilent manuals, page 137

  • Agilent Effect of Bonded-Phase on Resolution Retention of Polypeptides

    The document discusses the effect of bonded-phase on the resolution and retention of polypeptides. It highlights that differences in bonded-phase selectivity can significantly improve the resolution in reversed-phase analysis of peptides and proteins. The separation between peaks 2 and 3, peaks 5 and 6, and peaks 8 and 9 illustrate these selectivity changes. Additionally, it mentions that temperature can also be used to achieve desired separations. The steric protection of ZORBAX SB packings makes them well suited for chromatography at very low pH.

  • Agilent Technologies Effect of Injection Volume on Resolution in Size-Exclusion Chromatography

    This document describes how to use small sample injection volumes in size exclusion chromatography

  • Agilent Technologies Effect of FLow Rate on Resolution in Size Exclusion Chromatography

  • Agilent Technologies Effect of Column-Length on Efficiency Resolution

    This experiment report introduces the effect of column length on efficiency and resolution. When appropriate, two or more SEC columns may be connected in series to achieve a desired separation.

  • Agilent Technologies Effect of Mobile-Phase Ionic Strength on Neutral Strongly Basic Antibodies at pH 7.0

    This document investigates the effect of mobile-phase ionic strength on neutral and strongly basic antibodies. The results show that strongly basic molecules interact with silica-based column packings to a much greater extent than neutral molecules. Additionally, the concentration of phosphate buffer has an impact on peak shape and retention time.

  • Agilent Technologies Effect of Elevated Temperatures on RP HPLC of Highly Hydrophobic Peptides

  • Agilent Technologies High-Resolution Chiral Separation of Fluoxetine (PROZAC ) Enantiomers Using ULTRON ES-OVM

    The document introduces a high-resolution chiral separation technology for Fluoxetine (Prozac) enantiomers using the ULTRON ES-OVM column

  • Agilent Technologies Fast Separation of IgM AND IgG Antibodies by Size-Exclusion Chromatography

    This document introduces a method for separating immunoglobulins using size-exclusion chromatography. The method uses a high-performance ZORBAX particle that is extremely rugged, even under high back-pressures. This characteristic allows higher flow rates and shorter run times for high-throughput experimentation. The method can also reduce the injection volume of an antibody sample without loss of sensitivity, when using smaller-diameter columns.

  • Agilent Technologies ZORBAX GF-250 Separation of Bio-Rad Gel-Filtration Standards

    This document introduces ZORBAX GF-250 column in the separation of Bio-Rad molecular weight markers

  • Agilent Calibration of a ZORBAX GF-250 Column Using Bio-Rad Gel-Filtration Standards

    This document describes how to calibrate a ZORBAX GF-250 column using Bio-Rad gel filtration standards.

  • Agilent High-Resolution Separation of the Human Amyloid Aß peptides - Absorbance (210 nm)

    This document describes the complete separation of ß-amyloid peptides (ßAP) using Agilent ZORBAX 300 SB-C8 column. The inset box shows the high recovery following the HPLC separation, as determined by direct protein assay of collected peaks. The separation of these peptides was not possible at column temperatures below 80°C. High-resolution separation of human amyloid Aß peptides highlights: • Sterically-protected bonded phases (StableBond) permit use of low pH mobile phases, even at high temperature. • Use of elevated column temperature can dramatically improve separation and recoveries of difficult samples. • Note the greater retention of ßAP(1- 42) than ßAP(1-43). Application Biochemical Robert Ricker Conditions: ZORBAX 300 SB-C8, 4.6 mm ID x 250 mm, Agilent Part No. 880995-906 Mobile Phase: 80°C; 0.1% TFA in 30.5% acetonitrile / 69.5% water 2.5 µg each peptide in 20 µL 6 M urea / 5% HOAc Flow: 1 mL/min. Absorbance (210 nm) ßAP(1-38) ßAP(1-43) ßAP(1-40) ßAP(1-42) Time (min.) Rs = 2.52 0 4 8 12 16 20 Recovery (% ± S.D., n = 3) 1-38 111 ± 14 1-40 96 ± 14 1-42 75 ± 10 1-43 69 ± 10 Copyright© 2002 Agilent Technologies, Inc. All Rights Reserved. Reproduction, adaptation or translation without prior written permission is prohibited, except as allowed under the copyright laws. For more information on our products and services, visit our website at: www.agilent.com/chem Robert Ricker is an application chemist based at Agilent Technologies, Wilmington, Delaware. Agilent shall no

  • Agilent Monitoring the Purification of Synthetic Amyloid Peptide Ab (1-42) by High Temperature RP-HPLC

    This is an example of using high temperature reversed-phase liquid chromatography to separate and purify amyloid peptide ab (1-42). This method takes advantage of isocratic separation (no re-equilibration time) using highly efficient 3.5 µm particle diameter, 300Å pore size, Agilent ZORBAX SB-C18 column packing material. High temperature separation enhances recovery and improves peak shape for this badly behaved peptide.

  • Agilent Biogenic Amines Catecholamines

    The document describes a method for high-performance liquid chromatography (HPLC) analysis using ion-pair reversed-phase separations combined with electrochemical detection to measure the levels of catecholamines and indoleamines in brain tissue. The method is rapid, selective, and efficient, allowing for analysis of samples in less than 10 minutes per sample. The use of a highly stable column-packing material (Agilent ZORBAX SB-C8, 3.5 µm) ensures unmatched column lifetime, enabling analysis of thousands of samples per column. Samples are directly injected as perchloric acid (0.1N) extracts, eliminating the need for sample workup. This method is suitable for the determination of various biogenic amines neurotransmitters and their metabolites.

  • Agilent Improved Throughput of deoxyNucleosides Using New Column Configurations

    The document describes the common analysis method for deoxynucleosides and presents a rapid analysis method suitable for LC-MS. The method utilizes Agilent ZORBAX SB-CN column for separation, providing good selectivity and resolution.

  • Agilent Separation of rhGH Tryptic Digest Fragments on a 1 mm ID Column

    This document introduces the experimental method of separating the recombinant human growth hormone trypsin digestion fragments by using Agilent ZORBAX 300SB-C18, 5µm, 1 mm x 150 mm microbore column.