SB-C3 is a shorter-chain bonded phase that is less retentive than SB-C8 but more retentive than a non-sterically protected TMS phase. SB-C3 can be used to decrease solute retention and is more stable than the non-sterically protected TMS phase.
This technical application note compares the column retentivity of Agilent ZORBAX SB-C8 and SB-C3 bonded phases against Triton X-114. The analysis indicates that the SB-C3 phase is less retentive than the SB-C8 phase but more retentive than a C-1 phase.
| Column Dimensions | 4.6 x 150 mm |
|---|---|
| Mobile Phase | Methanol/H2O |
| Flow Rate | 1 mL/min |
| Temperature | 50°C |
| Detection | UV( 254 nm) |
SB-C3 is less retentive than SB-C8.
SB-C3 is more retentive than a C-1 phase.