This Application Note illustrates how to isolate and purify reaction byproducts using the Agilent 1100 Series purification system (AS) and demonstrates that large-scale pooling can be elegantly facilitated by incorporating the funnel tray into the Agilent 1100 Series fraction collector AS.
The document describes how the Agilent 1100 Series purification systems (analytical scale AS and preparative scale PS) can automate the isolation of reaction byproducts, a common task in the pharmaceutical industry. It highlights the use of large-scale pooling to collect minor components from multiple injections.
| Flow rate (Preparative) | 6.0 mL/min |
|---|---|
| Injection volume (Analytical) | 20 ul |
| Column temperature (Preparative) | ambient |
| Detector wavelength | 254/4 nm |
The funnel tray overcomes volume limitations of standard vials and allows fraction collection in almost any kind of vessel.
40 consecutive chromatographic runs were performed.