Agilent Manuals

This document describes how to use high-performance liquid chromatography (HPSEC) to monitor differences in synthetic polymer production. Specifically, the material uses the ZORBAX Bimodal S column (Agilent P/N: 880957-814) to perform size exclusion separations and uses ultraviolet detection (UV 254 nm) to detect the quality of polymethacrylate materials.

The document describes the features and characteristics of commercial polyethylene, including the requirement of elevated temperature and aggressive solvent systems for high-performance size exclusion chromatography (HPSEC). It also highlights the stability of ZORBAX PSM size exclusion columns with aggressive solvent systems. The document provides detailed analysis conditions and information about the application in polymers. It is authored by Robert Ricker, an application chemist at Agilent Technologies.

This document describes a method for rapid quality testing of uncured epoxy resins. The method utilizes HPSEC instrument with the following conditions: column: DuPont HPLC ZORBAX PSM 60S, 6.2 mm x 25 cm (Agilent P/N: 880957-802); mobile phase: tetrahydrofuran; flow rate: 1.5 mL/min; temperature: 22°C; detector: UV Abs (254 nm); sample: Epon 836.

The document introduces a stable C18 column that can be used for high temperature separation of low molecular weight polymers

This document describes the features of ZORBAX StableBond C8, which has a sterically protected bonded phase that allows stable operation at low mobile-phase pH. ZORBAX SB-C8 separates basic compounds with good resolution and peak shape using simple mobile phases. The document also provides the conditions for using ZORBAX Rx/SB-C8 in pharmaceutical applications.

This document describes that Agilent Technologies' Rx-Silica highlights good peak shape of basic compounds in opiate drugs. These basic compounds have poor peak shape on many reversed-phase columns. ZORBAX Rx-SIL has an extremely homogeneous silica surface with uniform associated silanols and low acidity. ZORBAX Rx and StableBond columns show significantly reduced band tailing and improved column efficiency when analyzing basic compounds.

This document describes a method for separating local anesthetics on different bonded-phase ZORBAX columns and analyzes the selectivity of the separations.

This document describes the separation performance of ZORBAX XDB-C8 for Procainamides and metabolites at a pH of 7.0 or higher. It provides good peak shape and resolution, making it suitable for pharmaceutical applications.

This document describes the separation of cephalosporins on Agilent ZORBAX XDB-C8 column.

This document describes a method for high-resolution chiral separation of R- and S-Norfluoxetine using Ultron ES-Pepsin. The method achieves excellent separation and extends the column's useful lifetime.

The document describes the separation method for extracting morphine and its metabolites from blood plasma. Good resolution and peak shape for morphine and two metabolites can be achieved using ZORBAX SB-C18 at moderate pH. Morphine and the metabolite morphine-6-glucuronide can be sensitively detected using electrochemical detection, while morphine-3-glucuronide can be sensitively detected using fluorescence.

This document describes the separation method for tricyclic antidepressants and their metabolites. It explains that good peak shapes can be obtained for very basic compounds at pH 6.2 by adding TEA. The document also suggests that changing the pore-size and surface area of the column packing can help achieve the desired separation. Specific analysis conditions and methods are provided.

This document describes the high-resolution separation method and characteristics of nonsteroidal anti-inflammatory drugs, as well as the narrow-bore column configuration suitable for LC/MS applications. The Eclipse XDB-C8 column is capable of separating acidic, basic, and neutral molecules with good peak shape. Specific analysis conditions and the pKa values of the drugs are provided.