Agilent 1100 Series Purification System Application Note: Isolation of Formononetin

This application note describes how to isolate active natural products from red clover using the Agilent 1100 Series purification systems.

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 8
File format: PDF
File size: 651 KB
Published: 03 July, 2012
Updated: 30 September, 2023
Model: 1100 Series
MD5 checksum: 4F52A678F84F42DBAF8475C07C1F2761
PDF: Agilent 1100 Series Purification System Application Note: Isolation of Formononetin PDF

About this manual

This document describes a method for isolating formononetin and other phytoestrogens from red clover extracts using the Agilent 1100 Series purification systems. The process utilizes both analytical scale (AS) and preparative scale (PS) configurations to separate these compounds effectively.

What's inside

Introduction
Equipment
Extraction
Analytical method development
Volume overloading experiment
Isolation of phytoestrogens in analytical scale
Isolation of higher amounts
Isolation of phytoestrogens in preparative scale
Conclusion

Specifications

Mobile Phase A	0.1 % HOAc in water
Mobile Phase B	0.1 % HOAc in acetonitrile
Flow Rate (Preparative)	7 ml/min

Frequently asked questions

What systems were used for the experiments?

The experiments used the Agilent 1100 Series vacuum degasser, quaternary pump, well-plate autosampler, thermostatted column compartment, diode array detector, and fraction collector AS for analytical scale, and preparative pumps, autosampler, column organizer, and fraction collector PS for preparative scale.

What is the flow rate for the preparative scale experiment?

The flow rate for the preparative scale experiment on the 9.4 x 150 mm column is 7 ml/min.

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