This document describes how to improve separation by taking advantage of different bonded-phase chemistries. When using StableBond sterically protected phases, both long-chain and short-chain bonded phases are stable.
The document presents an application demonstrating the effect of bonded phase chemistry on the selectivity and separation of ten specific polypeptides. It compares the performance of four different stationary phases: ZORBAX 300SB-C18, C8, C3, and CN.
The study utilizes a reversed-phase chromatography method with specific conditions including a mobile phase gradient from 25% to 70% organic modifier (ACN) over 40 minutes. The analysis was performed at 60°C using UV detection at 210 nm.
The mobile phase gradient runs for 40 minutes.