Agilent Manuals

This application notes updates the USP methods for metronidazole to improve column efficiency and sensitivity.

The document describes the method and advantages of using ZORBAX Eclipse XDB-C18 for HPLC analysis. By using ZORBAX Eclipse XDB-C18, improved peak shape, higher separation efficiency, greater sensitivity, reduced analysis time, reduced back pressure, and reduced solvent use per analysis can be achieved. The performance of this product surpasses the requirements of USP, with a higher number of theoretical plates and a closer tailing factor to the ideal value.

This is an application note on the use of tetracycline on the Agilent ZORBAX Rx-C8 column, including peak shape and resolution.

This document describes how new technologies can achieve excellent peak shape in the separation of tricyclic antidepressants using the Agilent XDB-C8 column and a mobile phase containing THF.

The document describes the USP method for analyzing Buspirone in pharmaceutical applications using HPLC. It highlights the use of ZORBAX Eclipse XDB-C18 column for improved peak shape and higher efficiency.

This application note describes a HPLC method for the analysis of methotrexate. The method uses a 10/90 acetonitrile/pH 6.0 phosphate/citrate buffer mobile phase and a ZORBAX Eclipse XDB-C18 column, column dimensions 3.9 x 300 mm, particle size 10 µm.

This document describes the analysis of Nortriptyline in plasma. It utilizes on-line extraction and separation using a ZORBAX Eclipse XDB-C8 column, which provides excellent peak shape. The method operates optimally over a wide pH range. This fast and direct approach is used to determine physiological levels of Nortriptyline in the bloodstream.

The document describes a method for analyzing carbamazepine and phenytoin in plasma. The analysis is performed using online extraction and a ZORBAX SB-CN column, allowing for fast and direct analysis of these anticonvulsant drugs.

This document describes the analysis of Prazosin in serum and highlights its features and advantages in pharmaceutical applications.

This document introduces the LC/MS detection method of LSD.

This document describes a method for LSD detection using liquid chromatography-mass spectrometry. The method features rapid separation, good peak shape, and excellent sensitivity. The use of an internal standard, LAMPA, enables quantitative analysis of LSD. The method is applicable in the pharmaceutical field.

The document describes a method for high-speed separation of analgesics using smaller particles and column dimensions along with high temperature to resolve seven analgesics in less than one minute. The use of high temperature reduces run times. ZORBAX SB-C18 can operate at higher temperatures and lower pH than other commercial reversed phase columns.

This document describes the features of ZORBAX Bonus-RP, including its unique selectivity and peak shape, which significantly improves resolution between peak 2 and an impurity. In addition, ZORBAX Bonus-RP is a unique polar-linked bonded phase that provides good peak shape, improved quantitation and resolution, long life over a wide pH range, and is suitable for pharmaceutical applications.

The document discusses the advantages of using Agilent ZORBAX Rx-SIL reversed-phase HPLC column for the separation of amines, particularly in the analysis of cough syrups. By using a reversed-phase mobile phase, the excessive retention and poor peak shape caused by the interaction of amines with unbonded silanols can be overcome. The Rx-SIL particles are stronger and more resistant to dissolution compared to xerogel silicas. This method provides the desired separation for the compounds commonly associated with cough syrups.