This document describes the analysis method for ergosterol, a pro-vitamin of Vitamin D2, in soil extracts. Ergocalciferol (Vitamin D2) is produced by the ultraviolet irradiation of ergosterol in plants, fungi, and yeasts. The use of Agilent ZORBAX Rapid-Resolution Rx-C18 columns enables fast analysis of soil extracts with good peak shape and retention.
Ergosterol is a pro-vitamin of Vitamin D2, which is produced in plants, fungi, and yeasts by the ultraviolet irradiation of ergosterol. This document details a method for analyzing extracted soil samples containing ergosterol.
The method utilizes Agilent ZORBAX Rapid-Resolution Rx-C18 columns to achieve fast analyses with good peak shape and retention. The specific conditions include a mobile phase of water and methanol (2:98), UV detection at 280 nm, and a flow rate of 1.0 mL/min at ambient temperature.
| Column | ZORBAX Rx-C18, 4.6 x 75 mm (3.5 pm) |
|---|---|
| Mobile Phase | H2O : MeOH, 2:98 |
| UV Wavelength | 280 nm |
| Flow Rate | 1.0 mL / min. |
| Temperature | Ambient |
| Part Number | 866967-902 |
This application demonstrates the chromatographic conditions used for analyzing extracted soil samples.
The column used has dimensions of 4.6 x 75 mm.
The specified flow rate is 1.0 mL / min.