This document introduces a method for detecting the purity of synthetic �-homo polylysine (250) using capillary electrophoresis. Experimental results show that the purity of synthetic �-homo polylysine (250) in 50 mM phosphate buffer (pH 2.0) solution is greater than 99.7%.
This document details a purity check performed on a synthetic alpha-homo polylysine (250-mer) preparation using capillary electrophoresis. The analysis successfully resolved smaller synthesis by-products from the main component, estimating the purity level of the 250-mer to be greater than 99.7%.
| Buffer | 50 mM phosphate, pH 2.0 |
|---|---|
| Sample | synthetic polylysine 250-mer |
| Capillary effective length | 72 cm |
| Capillary total length | 80.5 cm |
| Capillary internal diameter | 50 um |
| Detection internal diameter | 150 um |
| Injection pressure | 200 mbars |
| Temperature | 25 °C |
| Purity level | > 99.7 % |
The purity level was estimated to be greater than 99.7%.
A 50 mM phosphate buffer at pH 2.0 was used.