Agilent ZORBAX SB-C8 Separation of Deoxy Ribonucleosides Application Note

This document introduces a high-efficiency method for separating nucleosides using reversed-phase separation with protected-bonded-phase columns.

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 1 MB
Published: 03 July, 2012
Updated: 30 September, 2023
Models: SB-C8, ZORBAX SB-C8
MD5 checksum: C98BFAE59A956962331FA6819D26D61E
PDF: Agilent ZORBAX SB-C8 Separation of Deoxy Ribonucleosides Application Note PDF

About this manual

This document details a high-speed, high-resolution separation method for nucleosides, specifically Cytidine, 2 Deoxycytidine, Inosine, Guanosine, 2'- Deoxyinosine, Adenosine, and 2'- Deoxyguanosine. The method utilizes reversed-phase separation with sterically protected bonded-phase columns to achieve good peak shapes and high column efficiencies.

What's inside

Biochemical
Separation of Deoxy and Ribonucleosides
Highlights
Conditions
ZORBAX SB-C8
Mobile Phase
Flow
Detection

Specifications

Column Part Number	866953-906
Column Dimensions	4.6 x 75 mm
Mobile Phase Composition	5% methanol, 95% phosphate buffer
Flow Rate	2.0 mL/min
Detection Wavelength	254 nm
Injection Volume	1.6 pg each

Frequently asked questions

What is the recommended column size for this separation?

The recommended column size is 4.6 x 75 mm.

What is the recommended flow rate for this separation?

The recommended flow rate is 2.0 mL/min.

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