Agilent Manuals

This Application Note briefly describes the advantages of mass-based fraction collection compared to time and peak based fraction collection in combination with the Agilent 1200 Series purification system and the Agilent 6100 Single Quadrupole LC/MS

This article introduces how to use Agilent 1200 series LC system for impurity analysis, and gives the structure elucidation method of impurities

Nanospray LC-MS analysis of dextromethorphan (DEM) and its metabolites was performed using the Agilent 1200 Series HPLC-Chip/TOF system. The high sensitivity TOF in combination with automated nanospray HPLC-Chips enabled the detection of all DEM metabolites at concentration levels 100-fold lower than normal. The system response was linear over a wide concentration range for metabolites even when spiked into human plasma and urine. The LODs for real-life samples were in the high attomol to low femtomol range. Sample preparation was simplified using acidic precipitation by perchloric acid and a short centrifugation step. The simplified sample preparation, accurate mass measurements, excellent chromatographic reproducibility, and easy-to-use nanospray chip system make this setup optimal for routine use, allowing for higher throughput and reduced sample preparation costs.

The document describes an improved method for analyzing opioids, cocaine, and cocaine metabolites from blood using solid phase extraction followed by LC/MS.

This work demonstrates that Eclipse Plus C18 can be used for high-performance liquid chromatography (HPLC) separation of difficult to separate nitrogen-containing compounds without the use of triethylamine (TEA), which provides a new method for separating such compounds. In addition, the low end-group activity of Eclipse Plus C18 makes it a preferred HPLC column for analysis of pharmaceuticals and other amine-containing compounds.

This application describes how RRHT columns allowed for the rapid development of methods for selected endocannabinoids.

This Application Note describes the performance of the system using a 7-peptide sample and a tryptic digest of four different proteins.

This report outlines preliminary methodology and results for the analysis of four samples for all three isotopes of sulfur in carbonate matrices using the 7500ce Octopole Reaction System (ORS) with xenon as the cell gas. Xenon was used to react with oxygen in order to attenuate the O2-derived polyatomic interferences at masses 32, 33, and 34. Consequently, each sulfur isotope was analyzed on-mass, leading to highly reproducible results.

This document discusses the risks of residual solvents in pharmaceutical products and the regulatory requirements from national and international bodies. The use of Agilent 7890A GC and Agilent G1888 automated headspace sampler allows for sensitive and accurate analysis of solvent residues. The document provides details on experimental conditions and instrument parameters.

This application note describes a method for analyzing antibody samples using an Agilent 1200 series HPLC coupled with a 6210 TOF MS. The results indicate that this method can quickly and accurately determine the molecular weight (MW) of antibodies and can resolve the various antibody subpopulations present in a sample.

This Application Note describes the analysis of different antibody sam- ples under reducing and non-reducing conditions.

This document is a compendium of HPLC analysis methods for traditional Chinese medicine and chemical drugs in the China Pharmacopoeia. It includes HPLC analysis methods for traditional Chinese medicine from Volume I and chemical drugs from Volume II of the 2005 edition of the China Pharmacopoeia. The document highlights the selectivity and sensitivity of HPLC as an analysis technique, as well as its widespread adoption in the China Pharmacopoeia.

This document introduces Agilent's GC and LC column selection guides, which make it easier for customers to select the right columns using simple guides. Comprehensive column choices improve lab productivity, reduce method development time, and improve results.

This document introduces the use of the Agilent 1200 Series liquid chromatography (LC) system coupled to the 6410 Triple Quadrupole Mass Spectrometer (QQQ) by way of the G1948B electrospray ionization (ESI) source, for the analysis of anabolic substances in urine.

This Application Note describes the use of a high-throughput rapid resolution LC/MS TOF system for detection of impurities in a pharmaceutical formulation in more than 1000 samples per day. The instrument setup and a method for alternating column regeneration in a high-throughput LC system to reduce the overall analysis time by 50 % are also described.