Agilent Manuals

The document compares the performance of Agilent Technologies' new series of instruments, the 7890 Series GC and 5975 Series MSD, to the previous versions, the 6890 Series GC and the 5973 Series MSD. Performance is shown to be fully equivalent in test comparisons using a 16-component polynuclear aromatic hydrocarbon (PAH) sample. The document also discusses the reliable transfer of existing methods from the 6890/5973 system to the new 7890/5975 system.

Using an Agilent 1200 Series LC system, featuring the G1316B Thermostatted Column Compartment, is used to generate reproducible chromatography with a programmable tem- perature gradient

This application note describes the use of the Agilent 1100 Series LC system for the analysis of β-blockers in blood and other biological fluids using online and offline sample preparation methods.

The Agilent 1200 Series Rapid Resolution LC system can operate continuously to meet the stringent requirements of multiple medicinal chemists for a high-throughput LC/MS system.

Using two identical columns and a second pump can achieve high-throughput analytical HPLC.

Optimizing the Agilent 1100 Series well-plate sampler

Microfluidic chips for detecting microsatellite instability

This Application Note presents a workflow for the identification of main drug metabolites by sample comparison as well as by structure elucidation of the identified metabolites based on accurate MS and MS/MS data acquired with a quadrupole time-of-flight (Q-TOF) LC/MS. In pharmaceutical drug development the main metabolites of a new drug candidate must be identified as fast as possible and with high confidence. Software for this task is described, which analyzes the metabolized drug samples and visualizes the differences with non- metabolized drug samples for manual investigation. The structures of possible metabolites are elucidated by examination of the original highly-accurate Q-TOF mass data based on the differences between the metabolized and non-metabolized drug samples.

This application note describes how to use Agilent’s Tiling Microarray Technology to monitor genome-wide methylation patterns in CpG islands.

This document describes the development of a rapid resolution liquid chromatography (RRLC) method for the analysis of notoginseng. The method has good resolution and can be used to quickly analyze complex components, thereby increasing sample throughput and lowering costs. The method can also be used to analyze compounds in different parts of the notoginseng plant, and different peak profiles and different concentrations of certain saponins are detected.

This article describes the optimization of selectivity for antibody separations using HPLC and LC/MS by increasing the percentage of n-propanol and acetonitrile in 0.1% trifluoroacetic acid using ZORBAX 300 Å SB-CN LC Columns.

This article introduces the techniques for isolating metabolites from biological samples using liquid chromatography with sub-2-micron particle columns. The method improves the rate and resolution of metabolite separation without compromising the overall purity or recovery of the metabolites.

This Application Note describes: • The rapid development of an MRM triple quadrupole MS method for compound quantification. • The fast liquid chromatography separation on 1.8 µm RRHT columns for high throughput of DMPK samples. • The application of the developed LC/MS MRM method for the quantification of compounds in a mixture by a multi MRM method.

This Application Note is based on scientific poster MOP 253 presented at the ASMS conference of mass spectrometry, June 4, 2007, in Indianapolis, Indiana, USA. The note describes: - Rapid, computer-assisted identification of drug metabolites using a multi-algorithm approach. - High resolution chromatographic separation of drug metabolites from an in vitro experiment. - High mass accuracy measurement of drug metabolites by QTOF MS and MS/MS.

This Application Note describes the rapid development of a MRM method for triple quadrupole mass spectrometry, the rapid development of a liquid chromatography method for high throughput conditions with a 1.8 µm RRHT column, the use of the developed LC/MS MRM method for rapid determina- tion of a metabolic stability profile of a pharmaceutical drug, and the relative quantification of the acquired data to generate the metabolic stability profile.