This application note describes the preparative isolation of corticosteroid degradation products using mass-based fraction collection.
The document describes a study on the preparative isolation of corticosteroid degradation products. It highlights that combining the Agilent Prep-C18 Scalar column with Series 1100 mass-based fraction collection software allows for the successful isolation of purified fractions from degraded mixtures.
The text details the experimental methods, including the use of an Agilent 1100 binary HPLC system and LC/MSD. It presents results showing that mass-based fraction collection enables a high degree of purity even under incomplete separation conditions, allowing for the characterization of degradation products via unique mass spectra.
The study used a sample concentration of 50 mg/mL for preparative runs.
An injection volume of 50 uL is used for preparative runs.