This article introduces a rapid protein identification technique using atmospheric pressure-matrix assisted laser desorption/ionization (AP-MALDI) time-of-flight (TOF) mass spectrometry.
This document provides a technical overview of the Agilent Spectrum Mill MS Proteomics Workbench, a software solution designed for high-throughput protein identification. It details the workflow for using Peptide Mass Fingerprinting (PMF) with the Agilent AP-MALDI source and LC/MSD TOF instrument to analyze peptide digests rapidly and accurately.
The text describes the experimental setup, including sample preparation and specific instrument conditions for the Agilent LC/MSD TOF. It outlines the software's capabilities for data extraction, PMF searching against databases like SwissProt, and results summarization, demonstrating the system's ability to identify proteins from mixtures with high accuracy.
| Mass accuracy (mean error) | -0.98 ppm |
|---|---|
| Analysis rate | one per minute |
| Database | SwissProt |
| Enzyme | Trypsin |
| Minimum matched peptides | 5 |
The mean error is -0.98 ppm.
The SwissProt protein database is used.