Agilent manuals, page 152
Agilent Proteome Profiling by Multidimensional Protein Separation Automated Lab-on-a-Chip Technology
Agilent Detection of a point mutation in the prothrombin gene with the Agilent 2100 bioanalyzer Application Note
This application note describes how the Agilent 2100 bioanalyzer can be used for the detection of a point mutation in the human prothrombin gene. The prothrombin protein plays a key role in blood clotting. A single nucleotide polymorphism (SNP), i.e. a point mutation in the prothrombin gene results in a common hereditary predisposition to venous thrombosis. We present a convenient and reliable mutation detection method by a PCR-based assay including a specific restriction digest (PCR-RFLP). Due to its high sensitivity and resolution, the applied microfluidic assay proves to be extremely suitable for detec- tion of the obtained PCR products and restriction fragments. The developed method is suitable for the workflow at Laborigo molecular diagnostic laboratory.
Agilent The 5975 inert MSD Benefits of Enhancements in Chemical Ionization Operation Technical Note
The 5975 inert MSD – Benefits of Enhancements in Chemical Ionization Operation Technical Note This Technical Note describes the complete integration of chemical ionization (CI) on the 5975 inert MSD (mass selective detector), which now makes all the power of CI mass spectrometry (MS) conveniently available. CI has been fully automated by providing auto-tuning with electronic control of the reagent gases. All modes of MS operation in a single sequence, completely free of operator intervention, are now possible. Analytes can be sensitively determined in samples by implementing alternating positive- or negative-CI (PCI or NCI) modes. On the 5975 inert MSD, CI is as easy as EI (electron impact ionization).
Agilent Quantitative Analysis of Opiates in Urine Using Accurate Mass LC/MSD TOF Application
This article presents a method for quantitatively analyzing opiates in urine using liquid chromatography/mass selective detector time-of-flight (LC/MSD TOF). The method utilizes accurate mass measurement to enhance selectivity.
Agilent Enhansing Lab Productivity with the Agilent ChemStation Data Browser Application Note
Agilent Rapid Accurate Sensitive Reproducible Analysis of Amino Acids Application Note
This is a document that introduces the features of the product.
Agilent Analysis of Clozapine: Zorbax SPE C18 EC Cartridge Application Note
This document describes the analysis method for the pharmaceutical compound clozapine, used for the treatment of severely ill schizophrenic clients. The use of Zorbax SPE C18 EC cartridge significantly reduces levels of interfering substances in the bloodstream, allowing for accurate quantification of clozapine or clozapine-like compounds.
Agilent Technologies Developing an eMethod for the Analysis of Volatile Organic Compounds in Water Using Purge Trap/GC with Agilent s New 5975 inert Mass Spec
This application note describes a method for the analysis of volatile organic compounds (VOCs) in water samples according to US Environmental Protection Agency (USEPA) method 8260B.
Agilent The 5973N-Inert MSD: Utilizing higher ion source temperatures. Application Note
This article introduces the new features of Agilent Technologies, Inc.'s 5973N inert mass spectrometer detector and ChemStation software, which allows for operation of the ion source at higher temperatures, providing improvements in analysis. It also provides advice and examples on how to explore the utility of ion source temperature.
Agilent Validated Multi-Residue Method for Extraction Analysis of Trace-Level Pesticides in Surface Water
A USGS analytical method using gas chromatography/mass selective detector (GC/MSD) was modified and used to identify the source of pesticides and to calculate the pesticide load in river water during storm seasons. The method was validated to cover 12 organophosphate pesticides and 5 pyrithroids. The limits of quantitation for these target compounds are 10–500 ppt using a GC/MSD selected ion monitoring (SIM) method.
Agilent Analysis of Active Compounds using the New Highly Inert HP-5Msi Column
The use of a highly inert capillary column can facilitate the quantitation of low levels of very active compounds. The use of the highly inert HP-5MSi column in conjunction with the Agilent 6890/5973 inert gas chromatograph/mass spectrometer (GC/MS) system allows the measurement of pesticides and environmental contaminants at low levels while maintaining response linearity over a wide concentration range.
Agilent The Determination of Residual Solvents in Pharmaceuticals Using the Agilent G1888 Network Headspace Sampler
The document introduces a method for determining residual solvents in pharmaceuticals using Agilent G1888 Network Headspace Sampler.
Agilent Combined EI CI Using a Single Source
This document introduces the optimized sources for electron ionization (EI) and chemical ionization (CI) of Agilent 5973x gas chromatograph/mass selective detectors (GC/MSDs). It also demonstrates how to acquire high-quality EI spectra with the CI source without changing the sources. The experimental results show that using the CI source can obtain comparable major and minor peaks to the EI source.
Agilent Determination of the Vasodilator Isosorbide-5-Mononitrate in Human Plasma using GC/MS with Electron Capture Negative Ion Chemical Ionization
This document introduces a GC/MS with ECD method for determination of isosorbide-5-mononitrate in human plasma.
Agilent Detection of Toxic Industrial Compounds: A Guide to Analytical Techniques