The present invention discloses a new type of liquid chromatography column, which has a short column length and small particle size, and can achieve efficient high-throughput analysis.
This document describes how to increase productivity in liquid chromatography (LC) and LC/MS analyses by using short columns with smaller particle sizes, specifically the ZORBAX Rapid Resolution High Throughput (RRHT) series. The text explains that maintaining resolution while reducing column length allows for significantly shorter run times.
The method requires a resolution greater than 3.0 for triamcinolone and the internal standard hydrocortisone.
The 30 mm column provides 8 times the sample throughput compared to the original 250 mm column.
The method uses a mobile phase composed of 60% methanol and 40% water.