Accurate mass measurements can be used to both confirm the presence of a compound and help identify unknowns. Obtaining accurate mea- surements using mass spectrometry has typically required highly skilled operators doing interactive instrument operation and data manipula- tion. This Application Note describes how using the Agilent LC/MSD TOF and new software for walk-up operation, chemists can get the accurate mass information they need easily and without much training.
Accurate mass measurements are typically performed by skilled operators using interactive instrument operation and data manipulation. This document explains how the Agilent LC/MSD TOF, combined with new software, allows chemists to obtain accurate mass information easily and without extensive training in a walk-up environment.
The Agilent Easy-Access software, adapted from the LC/MSD Quadrupole, simplifies operation by allowing users to log in, input sample information, and select pre-set methods. The system automatically handles calibrant delivery and internal reference mass correction, enabling any operator skilled enough to run a single quadrupole instrument to obtain accurate mass spectra. Users can receive automated reports via email or printout.
The submitter logs in with their name, selects a pre-set method, and inputs the target formula if empirical formula confirmation is desired.
If enough time has passed since the last run, the system automatically introduces a calibrant mixture and recalculates mass coefficients.
The system produces an Empirical Formula Confirmation report that indicates whether a compound is present based on calculated mass error.