This Application Note describes the configuration and set up of a purification system for the isolation of radiolabeled drug metabolites from biological samples applied by an injection pump system. Various fraction collection options, for example, time-based fraction collection monitored by a radiochemical detector or peak-based fraction collection on the radiochemical detector signal are described.
This document details the setup of a purification system designed to isolate radiolabeled drug metabolites from complex biological matrices like urine. The system utilizes an Agilent 1100 Series purification platform equipped with specific modules, including preparative pumps, a dual-loop autosampler, and fraction collectors.
| Flow rate | 4 ml/min |
|---|---|
| Stop time | 55 min |
| Injection volume | 10 mL |
| Column temperature | ambient |
| DAD wavelength | 254 nm |
The document describes a configuration of the Agilent 1100 Series purification system equipped with an injection pump system.
Fractions are collected either by time-based fraction collection or by peak-based fraction collection on the signal from a radiochemical detector.