The document describes the features and characteristics of some plant growth regulators, including the types and mechanisms of plant hormones.
This document serves as an application brief for the rapid analysis of plant hormones using High-Performance Liquid Chromatography (HPLC). It details the separation of twelve specific plant hormone compounds, highlighting the utility of gradient elution to reduce runtime and increase throughput for research into plant metabolism and disease mechanisms.
The recommended method utilizes an Agilent ZORBAX SB-C8 column (4.6 x 75 mm) with a Hewlett Packard 1050 LC system. The separation is achieved using a gradient of water with 0.1% TFA and acetonitrile with 0.1% TFA, with the organic modifier increasing from 22% to 50% over 1.5 minutes. The analysis is performed at 60°C with a flow rate of 3.0 mL/min and detection at 254 nm.
| Column | ZORBAX SB-C8, 4.6 x 75 mm |
|---|---|
| Column Part Number | 66953-906 |
| Mobile Phase A | Water with 0.1% TFA |
| Mobile Phase B | Acetonitrile with 0.1% TFA |
| Gradient | 22% B to 50% B in 1.5 min |
| Wavelength | 254 nm |
| Flow Rate | 3.0 mL / min |
The method separates twelve plant hormones including Kinetin, Indole Acetic Acid, and various chlorophenoxy acids.
The document specifies a column size of 4.6 x 75 mm.
The procedure requires using a temperature of 60°C.
The application note states a flow rate of 3.0 mL/min.