This document introduces the HPLC analysis method for androgen drugs
The document presents a High-Performance Liquid Chromatography (HPLC) method for analyzing two androgen drugs: testosterone and testosterone acetate. The method utilizes a reversed-phase column with UV detection at variable wavelengths to separate the compounds effectively.
The analysis is performed using an Agilent 1100 Series system, which includes a quaternary pump, thermostatted autosampler, thermostatted column compartment, and a variable wavelength detector. Specific performance metrics such as Limit of Detection (LOD), retention time precision, and area precision are provided for both compounds.
| Column dimensions | 4.6 x 125 mm Hypersil ODS, 5 um |
|---|---|
| Mobile phase A | water |
| Flow rate | 1.0 ml/min |
| Column temperature | 40 °C |
| Injection volume | 5 ul |
The method uses an Agilent 1100 Series system including a quaternary pump, thermostatted autosampler, and variable wavelength detector.
The recommended column is Hypersil ODS, 5 um, with dimensions of 4.6 x 125 mm.
The LOD for testosterone is 0.25 mg/l based on a signal-to-noise ratio of 2.