This document introduces the application of HPLC technology in the analysis of hypnotic drugs, including separation methods, instrument conditions, etc.
The document presents a High-Performance Liquid Chromatography (HPLC) method developed specifically for the Agilent 1100 Series system to analyze eight specific hypnotic drugs: barbital, allobarbital, phenobarbital, butabarbital, butalbital, amobarbital, mephobarbital, and flunitrazepam. The method utilizes a reversed-phase column with UV detection at 204 nm.
| Column dimensions | 4x 125 mm |
|---|---|
| Particle size | 5 um |
| Mobile phase A | water |
| Mobile phase B | acetonitrile |
| Flow rate | 1.0 ml/min |
| Detection wavelength | 204 nm |
| Column temperature | 25°C |
| Injection volume | 5 ul |
The method analyzes barbital, allobarbital, phenobarbital, butabarbital, butalbital, amobarbital, mephobarbital and flunitrazepam.
The method was developed on the Agilent 1100 Series system including a quaternary pump, thermostatted autosampler, thermostatted column compartment, and variable wavelength detector.
The recommended guard cartridge is Hypersil ODS, 5 um, 4x 4 mm with part number 7992618-504.