This document introduces the conditions for analysis of anti-tumor drugs using the Agilent 1100 series HPLC systems.
This document details a High-Performance Liquid Chromatography (HPLC) method for the analysis of the antitumor agent paclitaxel (Taxol). The method utilizes a reversed-phase column and UV detection to separate and quantify the compound. Key parameters include a mobile phase gradient of water and acetonitrile, a flow rate of 1.0 ml/min, and a column temperature of 25 °C. The analysis is performed on an Agilent 1100 Series system equipped with a quaternary pump, thermostatted autosampler, and variable wavelength detector.
| Column | 4x 125 mm Hypersil ODS, 5 ym |
|---|---|
| Mobile phase A | water |
| Mobile phase B | acetonitrile |
| Flow rate | 1.0 ml/min |
| Column temperature | 25 °C |
| Stop time | 12 min |
| Injection volume | 5 ul |
| Precision of RT (RSD) | 0.07 |
| Precision of Area (RSD) | 0.79 |
| Correlation factor | 1.00000 |
The method uses an Agilent 1100 Series system with a quaternary pump, thermostatted autosampler, thermostatted column compartment, and variable wavelength detector.
The recommended column is Hypersil ODS, 5 um, 4x 125 mm (Agilent part number 7992618-564).
Yes, it is necessary to use a cooled autosampler, such as the Agilent 1100 Series thermostatted autosampler, to avoid sample decomposition.