Agilent 1100 Series Analysis of Quercetin and Kaempferol in Ginkgo Extract Application Note

This document describes the results of the separation of quercetin and kaempferol in ginkgo extract and tablets using HPLC.

Brand: Agilent
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 54 KB
Published: 03 July, 2012
Updated: 30 September, 2023
Model: 1100 Series
MD5 checksum: 48256FCC7B7FC37D1C87052E87AE4E69
PDF: Agilent 1100 Series Analysis of Quercetin and Kaempferol in Ginkgo Extract Application Note PDF

About this manual

The document presents a High-Performance Liquid Chromatography (HPLC) method developed on the Agilent 1100 Series system for separating and quantifying quercetin and kaempferol in Ginkgo biloba extracts and tablets. The analysis utilizes a reversed-phase column with UV detection at 370 nm.

What's inside

Analysis of Quercetin and Kaempferol
HPLC Conditions
Extraction Procedure
Equipment Requirements
Column Specifications
Spectral Comparison

Specifications

Column	4x 125 mm Hypersil ODS, 5 um
Mobile phase A	0.5 % H3PO4 in water
Mobile phase B	methanol
Flow rate	2.0 ml/min
UV detector wavelength	370 nm
Column compartment temperature	25°C
Injection volume	10 ul

Frequently asked questions

Can this method be used on older or newer systems?

Since the method was specifically developed on the Agilent 1100 Series system you might not be able to reproduce this analysis on an older system or even on a new system with lower performance.

What is the recommended guard cartridge?

The recommended guard cartridge is Hypersil ODS, 5 um, 4x 4mm (Agilent part number 7992618-504).

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