Agilent 1100 Series Analysis of Water-Soluble Vitamins by HPLC

This file introduces the application of HPLC in the analysis of water-soluble vitamins.

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 31 KB
Published: 03 July, 2012
Updated: 30 September, 2023
Model: 1100 Series
MD5 checksum: F0F44BDE72D6275CC0B93C5ED233B50C
PDF: Agilent 1100 Series Analysis of Water-Soluble Vitamins by HPLC PDF

About this manual

This document details a High-Performance Liquid Chromatography (HPLC) method for the analysis of eleven water-soluble vitamins, including pyridoxamine, thiamine, niacinamide, and others. The method utilizes gradient analysis on a reversed-phase column with UV detection at 204 nm.

What's inside

Analysis of Water-Soluble Vitamins
HPLC Performance
Equipment
Columns
Chromatogram Conditions

Specifications

Column	Zorbax SB-C18, 3.5 um, 4.6 x 75mm
Autosampler temperature	4°C
Flow rate	1.0 ml/min
Injection volume	5 ul
Detector wavelength	204 nm
Stop time	20 min
System	Agilent 1100 Series

Frequently asked questions

What equipment is required for this analysis?

The recommended equipment includes an Agilent 1100 Series Quaternary pump, thermostatted autosampler, thermostatted column compartment, and a variable wavelength detector.

What column is recommended for this analysis?

The recommended column is Zorbax SB-C18, 3.5 um, 4.6 x 75mm (Agilent part number 866953-902).

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