Agilent 1100 Series Analysis of Glucocorticoids by HPLC Application Note

This document describes the method of HPLC analysis of glucocorticoids.

Brand: Agilent
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 33 KB
Published: 03 July, 2012
Updated: 30 September, 2023
Model: 1100 Series
MD5 checksum: CD93DA701523041135E14308D4147A32
PDF: Agilent 1100 Series Analysis of Glucocorticoids by HPLC Application Note PDF

About this manual

The document details a High-Performance Liquid Chromatography (HPLC) method for separating and analyzing various glucocorticoid steroid hormones, including beclomethasone, hydrocortisone, prednisolone, and betamethasone. The method utilizes a reversed-phase column with UV detection at 254 nm.

What's inside

Analysis of Glucocorticoids
HPLC Conditions
Equipment List
Performance Data
Column Specifications

Specifications

Column	4x 125 mm Hypersil ODS, 5 um
Mobile phase A	water
Flow rate	1.0 ml/min
UV detector wavelength	254 nm
Column temperature	25 °C

Frequently asked questions

What is the injection volume?

The recommended injection volume for this method is 5 ul.

What is the purpose of setting the autosampler temperature to 4°C?

The autosampler temperature was set to 4°C to avoid decomposition of the samples.

At what wavelength does the UV detector operate for this analysis?

The variable wavelength detector operates at a standard cell setting of 254 nm.

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