Agilent 1100 Series Analysis of Atropine in Belladonna Extract by HPLC Application Note

This document introduces a HPLC method for the determination of atropine in Belladonna extract.

Brand: Agilent
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 45 KB
Published: 03 July, 2012
Updated: 30 September, 2023
Model: 1100 Series
MD5 checksum: 9B611CCBEEF6F6BF427EA5A5F31A93F3
PDF: Agilent 1100 Series Analysis of Atropine in Belladonna Extract by HPLC Application Note PDF

About this manual

The document details a High-Performance Liquid Chromatography (HPLC) method developed to analyze the tropane alkaloid atropine in extracts of Atropa belladonna. The method utilizes a reversed-phase column and UV detection, providing separation of atropine from other components in the plant extract.

What's inside

Analysis of Atropine
Extraction Procedure
HPLC Conditions
Equipment List
Column Specifications

Frequently asked questions

How is atropine extracted from dried and powdered belladonna plant material?

The sample is refluxed for 30 minutes in 0.5 M acetic acid, then the pH is adjusted to 9 before extracting five times with 50 ml chloroform.

What volume of extract should be applied to the HPLC system?

After filtration, exactly 5 pl of the prepared extract are applied to the HPLC.

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