Agilent 1100 Series Analysis of Testosterone Metabolites Application Note

This document introduces the detailed steps for analyzing testosterone and its metabolites using HPLC.

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 43 KB
Published: 03 July, 2012
Updated: 30 September, 2023
Model: 1100 Series
MD5 checksum: D79BB98E59FCD5031691A781724A5B9F
PDF: Agilent 1100 Series Analysis of Testosterone Metabolites Application Note PDF

About this manual

This document presents an application brief for the analysis of testosterone and its metabolites using High-Performance Liquid Chromatography (HPLC). The method utilizes a Cytochrome P450 (CYP) isoenzyme probe to demonstrate the capabilities of the system in detecting steroid compounds with high precision.

What's inside

Abstract
Conditions
Chromatogram
Analyzed compounds
Equipment
HPLC method performance

Specifications

Column	150 x 4.6 mm, 3 um
Mobile phase A	water
Column compartment temperature	40 C
Detector	diode-array detector
Wavelength	245 nm
Limit of detection	0.18 - 1.33 ng

Frequently asked questions

What software is required?

The system utilizes Agilent ChemStation software for data acquisition and analysis.

What is the limit of detection for the analyzed compounds?

The limit of detection ranges from 0.18 to 1.33 ng with a signal-to-noise ratio of 3.

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