The HPLC method presented here shows the chromatogram of the four common antiasthmatic drugs theophylline, theobromine, caffeine and emprofylline using isocratic analysis on a reversed phase column and UV detection.
This document presents a High-Performance Liquid Chromatography (HPLC) method developed by Udo Huber, an application chemist at Agilent Technologies. The method is designed to analyze four common antiasthmatic drugs: theophylline, theobromine, caffeine, and emprofylline.
The method analyzes theophylline, theobromine, caffeine, and emprofylline.
The autosampler temperature must be set to 4 °C.
The required flow rate is 1.0 ml/min.