Agilent 5973 CI MSD Application Note Determining Cannabinoids in Blood

About this manual

This document details a method for analyzing cannabinoids in biological matrices, specifically blood, using the Agilent 5973 CI MSD. The technique utilizes Electron Capture Negative Chemical Ionization (ECNCI) to detect and quantify Delta-9-tetrahydrocannabinol (THC) and its metabolite, 11-nor-9-carboxy-Delta-9-tetrahydrocannabinol (THCA). The method involves extracting these compounds from serum samples, derivatizing them to trifluoroacetyl derivatives (THC-TFA and THCA-TFA), and analyzing them via Gas Chromatography/Mass Spectrometry (GC/MS). The use of deuterated surrogates allows for accurate quantification with detection limits below 0.5 ng/mL.

What's inside

• Determining Cannabinoids in Blood
• Electron Capture Negative Chemical Ionization
• THC and THCA Analysis
• Derivatization Methods
• GC/MS SIM Parameters
• Calibration Curves
• Detection Limits

Specifications

Frequently asked questions

What are the molecular weights of the THC and THCA derivatives?

The trifluoroacetyl derivative of THC (THC-TFA) has a molecular weight of 410 amu, and THCA-TFA has a molecular weight of 454 amu.

What ionization method is used for this analysis?

The analysis uses Electron Capture Negative Chemical Ionization (ECNCI).