Agilent 6410 Triple Quadrupole Mass Spectrometer (QQQ) is used to analyze several water-soluble B-vitamin compounds in breakfast cereal, including thiamine, riboflavin, pyridoxine, vitamin B12, pantothenic acid, biotin, and niacinamide, which are present at relatively high levels. A simple gradient elution is carried out on a Rapid Resolution High Throughput SB-Aq column (particle size 1.8 µm). All compounds elute in less than 7.5 minutes, and with the exception of pyridoxine, good linearity over more than three orders of magnitude, from 0.5 to 500 ppb, is demonstrated with good peak area reproducibility at the 0.5 ppb level, which is the lowest level of quantitation considered.
The document details a quantitative analysis method for eight water-soluble B-vitamin compounds (Thiamine, Pantothenic acid, Pyridoxine, Nicotinic acid, Nicotinamide, Cyanocobalamin, Folic acid, and Riboflavin) in breakfast cereal. The method utilizes an Agilent 6410 Triple Quadrupole Mass Spectrometer coupled with an Agilent 1200 Series liquid chromatography system.
The method demonstrates good peak area reproducibility at 0.5 ppb, which is considered the lowest level of quantitation.
All compounds elute in less than 7.5 minutes with a simple gradient elution.
The method allows for screening and quantitation with a run-to-run cycle time as low as 10 minutes.