Analyzing a complex matrix can be accomplished using multiple specific detectors, but a significant time savings is realized using GC/MSD. Using an available Retention Time Locked database with Deconvolution Reporting Software (DRS) adds a second “expert” opinion. GC/MSD with DRS provides the fastest methodology to the fewest number of false positives/negatives with the greatest confidence in the results.
This document describes a methodology for reducing analysis time when analyzing complex matrices by using Agilent GC/MSD systems equipped with Deconvolution Reporting Software (DRS). The authors from Campbell Soup Company and Agilent Technologies explain how DRS combines results from MSD ChemStation Quantitation with AMDIS Deconvolution and NIST Search to provide an automated 'expert second opinion'.
The note details specific instrument operating parameters for an Agilent 6890N Gas Chromatograph and 5973N Mass Selective Detector, including a Programmable Temperature Vaporizing (PTV) inlet and HP-5MS column. It presents experimental results from analyzing six commodities (apples, lettuce, carrots, celery, green peppers, strawberries, and tomatoes) for pesticide residues, showing that DRS can identify trace quantities missed by standard quantitation methods.
| System Retention Time Locked to | methyl chlorpyrifos at 16.596 min |
|---|---|
| Sampling rate | 2 scans/sec |
| Scan range | 35-500 amu |
| NIST Search Library Size | > 163,000 compounds |
It automatically combines results from MSD ChemStation Quantitation with AMDIS Deconvolution and NIST Search into an easy-to-read report, directing the analyst to target compound hits without reviewing hundreds of possible compounds.
It maintains retention times after clipping or changing the column and allows multi-site laboratories to easily compare data.