Using RTL and 3-Way Splitter to Identify Unknowns in Strawberry Extract Application Note

About this manual

The document details a method for analyzing complex fruit and vegetable extracts, specifically strawberry extract. It utilizes a GC/MS system equipped with a three-way splitter that directs column effluent to two GC detectors (ECD and DFPD) and an MSD simultaneously.

A specific unknown peak was identified in the sample by combining retention time locking with element-selective detector chromatograms and searching the RTL pesticide database, successfully identifying the compound as temephos without relying on a full-scan mass spectrum.

What's inside

• Application Brief
• Three-Way Splitter System
• Unknown Peak Identification
• RTL Pesticide Database
• Temephos Confirmation

Frequently asked questions

What is the specific retention time window used to identify the unknown compound in the strawberry extract?

The analysis uses a 0.5-minute window centered at 41 minutes, which covers the range from 40.5 to 41.5 minutes.

Which compound was identified as the unknown peak based on the retention time and ion chromatograms?

The unknown peak was confirmed to be temephos, which has a target retention time of 40.74 minutes.

What are the four major ions used to confirm the identity of the temephos compound?

The confirmation uses target ion 466 and three qualifier ions with values of 125, 93, and 109.