The document introduces a method for authenticating basmati rice using the Agilent 2100 bioanalyzer and the DNA 1000 assay. This method is quick, cost-effective, and can be used to estimate the level of non-basmati rice in ground rice products.
This application note evaluates the use of the Agilent 2100 Bioanalyzer to differentiate approved and non-approved basmati rice varieties using three specific primer sets. The study aims to provide a quick and cost-effective analytical method to establish product authenticity and estimate the level of non-basmati rice in ground products, serving as an alternative to traditional methods.
The document details the experimental method, including DNA extraction using Qiagen or Promega kits, PCR amplification conditions, and capillary gel electrophoresis protocols. It presents results showing how the bioanalyzer resolves PCR products for various rice varieties and provides standard curves to estimate the percentage of non-basmati rice (specifically Pak 386) in admixtures.
| PCR Buffer | 1x Amplitaq Gold PCR buffer |
|---|---|
| Primer Concentration | 60 nM of each primer |
| dNTP Concentration | 200 nM dNTPs |
| Enzyme Concentration | 0.05 U/uL of Ampli-Tag Gold |
| Template Volume | 2 uL of template DNA |
| Denaturation Time | 95 °C for 15 minutes |
| Amplification Cycle Time | 95 °C for 1 minute, 60 °C for 1 minute |
| Extension Time | 72 °C for 10 minutes |
The level of non-basmati rice in a basmati rice product must not exceed 7%.
The study used Qiagen's DNeasy Plant Mini Kit or Promega's Maxwell 16 automated DNA extractor.
PCR products were loaded onto Series I DNA 500/DNA 1000 or Series II DNA 1000 labchips.
Yes, the bioanalyzer can be used to estimate the level of non-basmati rice in a basmati rice sample.