This document describes a high-speed HPLC method for separating parabens. The method uses a ZORBAX StableBond SB-C18 column and 0.1% H3PO4:ACN as the mobile phase. The run conditions are 2 mL/min flow rate, 1 µL injection volume, and 60℃ column temperature. The method can separate parabens in less than one minute.
This document details a high-speed analytical method for separating parabens, which are preservatives used in the cosmetic and food industries. The method achieves analysis times of less than one minute using a Rapid Resolution SB-C18 column.
The procedure utilizes an Agilent 1100 HPLC system with a ZORBAX StableBond-C18 Rapid-Resolution cartridge column (4.6 x 30 mm). Operating conditions include a mobile phase of 0.1% H3PO4 in ACN (50:50), a flow rate of 2 mL/min, and detection at 254 nm. Elevated temperatures (60°C) are used to further reduce run time.
| Flow Rate | 2 mL/min |
|---|---|
| Detection Wavelength | 254 nm |
| Analysis Time | Less than one minute |
The application note states that an analysis time of less than one minute is accomplished.
The operating conditions specify setting the bottom temperature to 60°C.