This document introduces a method for pesticide analysis using an Agilent J&W DB-35ms UI 20 m × 0.18 mm, 0.18 µm column, which can effectively separate organochlorine and organophosphorus pesticides with good separation and reproducibility.
This document details the analysis of challenging pesticide samples using the Agilent J&W DB-35ms Ultra Inert (UI) gas chromatography column. The note highlights how the column's inertness prevents peak tailing and degradation of active analytes like endrin and DDT, which are common issues in standard systems. It also emphasizes the column's midpolar phase for improved selectivity, allowing for the baseline resolution of coeluting peaks such as chlorpyrifos-methyl and methyl parathion.
| Column Dimensions | 20 m x 0.18 mm, 0.18 µm |
|---|---|
| Part Number | 121-3822UI |
| Carrier Gas | Helium |
| Flow Rate | 1.3 mL/min |
| Oven Temperature Program | 50 °C (1.3 min), 50 °C/min to 135 °C, 15 °C/min to 200 °C, 20 °C/min to 310 °C (2.5 min), 5 min at 310 °C |
The part number for the Agilent J&W DB-35ms UI column is 121-3822UI.
The Ultra Inert series helps minimize column activity so that difficult and active analytes can be consistently analyzed at trace levels without degradation.
The midpolar phase offers more selectivity to resolve difficult analytes, such as baseline-resolving chlorpyrifos-methyl and methyl parathion which coelute on 5% phenyl phases.