This application note describes how to use the SelecTemp™ and SelecFlow™ features on the Varian 325-MS triple quadrupole mass spectrometer to determine pesticides in a QuEChERS matrix.
This document details a method for determining pesticide residues in a QuEChERS matrix using the Varian 325-MS Triple Quadrupole Mass Spectrometer. The method utilizes the SelecTemp and SelecFlow features to optimize atmospheric pressure interface conditions, reducing source contamination and ion suppression while maintaining high-quality data acquisition. The setup includes a vESI source, a binary solvent delivery system, and an HTS PAL autosampler.
| Guard Column | Pursuit XRs C18, 3 um, MetaGuard 2.0 |
|---|---|
| Solvent A | 2 mM ammonium formate and 0.05% formic acid in water |
| Solvent B | Methanol |
The SelecTemp and SelecFlow features available on the 325-MS allow the analyst to modify the drying and vortex gas temperatures and pressures throughout an analytical run.
The divert valve allows eluent to be diverted away from the ionization source to reduce source contamination and minimize ion suppression.
The study evaluated Imazalil, Pirimiphos-methyl, Chlorpyrifos-methyl, and Chlorpyrifos.