This application note demonstrates how phospholipids can be identified in a mixture by triple quadrupole mass spectrometry and suspected structures confirmed by generating MS/MS spectra and submitting it to the ACD Labs/MS Manager software.
This document describes a method for identifying phospholipids in a mixture using triple quadrupole mass spectrometry. It details the use of the Varian 320-MS instrument equipped with an electrospray source and a built-in syringe infusion pump. The process involves preparing phospholipid standards (DPPE, POPG, and DPPG) and analyzing them via direct infusion.
| Collision Energy (DPPE) | 22V |
|---|---|
| Collision Energy (POPG) | 27 V |
| Collision Energy (DPPG) | 28 V |
| Infusion Flow Rate | 10 pL/min |
Samples are prepared as pure standards suspended at a concentration of 1 ppm in a 50:50 methanol:water mixture for direct infusion.
The samples were prepared by suspending them at a concentration of 1 ppm in a 50:50 methanol:water mixture.