This document describes a method for analyzing vulcanized rubber formulations using the Varian 500-MS ion trap mass spectrometer and confirming expected structures using Varian TurboDDS™ and ACD/MS Manager software.
This document details a method for analyzing vulcanized rubber extracts to identify polymeric components and specific chemical additives, such as Antioxidant 2246. The process involves liquid chromatography/mass spectrometry (LC/MS) to separate compounds and mass spectrometry/mass spectrometry (MS/MS) for structural elucidation.
The analysis utilizes the Varian 500-MS Ion Trap Mass Spectrometer equipped with TurboDDS software to automatically trigger MS/MS data collection for prominent peaks. The resulting data is then processed using ACD/MS Manager software to match predicted fragmentation patterns against experimental spectra, confirming the identity of suspected compounds.
| Column | Pursuit XRs C18, 3 pm, 150 x 3.0 mm |
|---|---|
| Mobile Phase | Acetonitrile |
| LC Flow Rate | 200 pL/min |
| LC Run Time | 30 min |
| Injection Volume | 20 pL |
| Nebulizing Gas | 35 psi |
| Drying Gas | 10 psi at 350 °C |
| Needle Voltage | 5000 V |
| Capillary Voltage | 80V |
| Shield Voltage | 600 V |
| RF Loading | 100% |
| Detector Voltage | 1900 V |
| Survey Scan Range | m/z 100-700 |
| MS/MS Trigger Threshold | 8600 counts |
| Minimum Intensity Ratio | 1% |
| Isotopic Equivalence | 3 m/z |
| Full Scan Range | m/z 100-1000 |
The suspected chemical component, Antioxidant 2246, has an m/z value of 341 for the protonated molecule.
This document describes how to analyze vulcanized rubber formulations and confirm expected structures using the Varian 500-MS Ion Trap Mass Spectrometer with TurboDDS software.
The liquid chromatography program uses an isocratic flow at 200 µL/min for a total run time of 30 minutes.
The survey scan function collects data over a mass-to-charge ratio (m/z) range of 100 to 700.