This document introduces the HPLC analysis method of muscle relaxant drugs, including instrument equipment, experimental conditions and results analysis.
The document presents a High-Performance Liquid Chromatography (HPLC) method specifically designed to analyze the muscle-relaxing drug papaverin. The procedure utilizes gradient analysis on a reversed-phase column with UV detection at 254 nm, ensuring reliable isolation of the compound from samples such as poppy or Rauwolfia species.
| Column dimensions | 4.6 x 75 mm Zorbax SB-C18 3.5 ym |
|---|---|
| Mobile phase A | 0.025 M KH,PQ, in water (pH = 3 with H,SO,) |
| Mobile phase B | acetonitrile |
| Flow rate | 1.0 ml/min |
| UV detector wavelength | 254 nm, standard cell |
| Column compartment temperature | 60 °C |
| Injection volume | 5 ul |
To avoid decomposition of the samples.
Agilent 1100 Series system including a quaternary pump, thermostatted autosampler, and variable wavelength detector.